NSure assays, always a step ahead

Long before plants transform visibly, changes in the activity level of specific genes have already taken place. By measuring these changes, valuable information is obtained. This information can be used to make decisions that are of great importance for a successful harvest or for optimal storage.  

Changes in environment
The natural development of plants is strongly influenced by changes in the environment. Examples of this are changes in the length of days, temperature and light intensity. Naturally, the application of certain treatments (chemical or otherwise) is also important. As a result of these changes, a fruit tree will make preparations during the winter to bloom in spring, and a pine seedling will build up cold tolerance during the autumn to survive the next winter.

The moment and rate at which such changes occur is highly dependent on the conditions which preceded the transformation. Thus, the bloom will be delayed in spring as a result of a mild winter and the storability of vegetables and fruit will be greatly affected by the level of invasive organisms prior to harvest.

Gene activity analysis

Because conditions vary greatly from year to year, it is almost impossible to make an exact estimate of the current phase of development of a plant or fruit based solely on visual or physiological examination. An analysis of the activity of genes, however, does give this insight. Furthermore, the technique also provides insight into the reaction of the plant to specific treatments. It therefore allows for a comparison between the effects of active substances, prototypes of commercial products, formulations and dosages.

Next Generation Sequencing
NSure uses Next Generation Sequencing (NGS) to examine the activity of all genes present in the plant at the same time. This provides a large amount of information. Our specific knowledge and expertise enables us to select those genes in which the activity is linked to a particular property such as bud dormancy, cold tolerance, resistance, disease pressure, drought stress, and so on. Once this selection has taken place, it is only necessary to pay attention to the activity of this specific set of genes, or indicators. These indicators are used for routine analyses that form the basis of our commercially available tests.